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Image Search Results
Journal: The Journal of Pharmacology and Experimental Therapeutics
Article Title: Intratracheal Administration of Acyl Coenzyme A Acyltransferase-1 Inhibitor K-604 Reduces Pulmonary Inflammation Following Bleomycin-Induced Lung Injury
doi: 10.1124/jpet.122.001284
Figure Lengend Snippet: Expression profile of surface markers used for flow cytometric analysis of alveolar and interstitial macrophages
Article Snippet: Immunomagnetic selection of CD45+ leukocytes was performed using the
Techniques: Expressing, Marker
Journal: The Journal of Pharmacology and Experimental Therapeutics
Article Title: Intratracheal Administration of Acyl Coenzyme A Acyltransferase-1 Inhibitor K-604 Reduces Pulmonary Inflammation Following Bleomycin-Induced Lung Injury
doi: 10.1124/jpet.122.001284
Figure Lengend Snippet: Flow cytometric analysis of macrophages isolated from lung tissue. Cells from digested lung tissue were immunomagnetically-separated based upon CD45 expression. CD45+ cells were isolated, immunostained, and analyzed ( Supplemental Fig 2 ). Cells that expressed F4/80 and CD11b in the absence of Siglec F were categorized as interstitial macrophages and were analyzed for CD11c/CD206 expression (A). The percentage of mature, chronically activated cells (CD11c+/CD206+, green box) was calculated from the total number of interstitial macrophages (B). Values are expressed as mean ± standard error of the mean ( n = 8-13 per group). (*) represents significantly different from control ( P < 0.05); (#) represents significantly different from ITB ( P < 0.05).
Article Snippet: Immunomagnetic selection of CD45+ leukocytes was performed using the
Techniques: Isolation, Expressing, Control